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Jul 31, 2023

Antioxidants From Mitochondria Protect Cells From Dying

Summary: The STARD7 enzyme helps transport the antioxidant coenzyme Q from the

Summary: The STARD7 enzyme helps transport the antioxidant coenzyme Q from the mitochondria to the cell surface.

Source: Max Planck Institute

Antioxidants are often advertised as a cure-all in nutrition and offered as dietary supplements. However, our body also produces such radical scavengers itself, one of which is coenzyme Q.

Now researchers from the Max Planck Institute for Biology of Ageing in Cologne, Germany, have discovered how the substance, which is produced in our mitochondria, reaches the cell surface and protects our cells from dying.

Coenzyme Q is an antioxidant that is essential for our body. A deficiency of coenzyme Q leads to serious diseases such as Leigh syndrome – a hereditary disease in which certain brain regions become affected and, among other things, muscle weakness can occur.

A deficiency of coenzyme Q is also one of the first signs of ageing and can occur as early as the early 20s. But why can't we simply take this substance in with our food?

"Coenzyme Q is a highly hydrophobic molecule that our bodies absorb very little from food," explains Soni Deshwal, scientist at the Max Planck Institute for Biology of Aging and lead author of the study. But it is also a problem in our cells that coenzyme Q is not water soluble.

The antioxidant is formed in mitochondria and must pass through the watery cell interior called cytoplasm to the surface of the cells in order to neutralize oxidized lipid species.

"With our research, we have now been able to identify the proteins involved in coenzyme Q transport from the mitochondria to the cell surface", explains Deshwal.

The researchers found that an enzyme called STARD7 helps transport the coenzyme. This protein is not only localized in the mitochondria, but also inside the cytoplasm.

"The mitochondria actively transport coenzyme Q to the cell surface to protect cells from cell death. It is as if the mitochondria deliver band-aids to the surface to protect the cell", says Deshwal.

"This again shows that mitochondria are not only important as an energy supplier for our cells, but also play crucial regulatory roles."

In the long term, the researchers hope that a precise understanding of this transport process will enable Coenzyme Q to be delivered into the cells of affected patients and thus provide a new therapeutic approach for diseases such as Leigh syndrome.

Author: Maren BerghoffSource: Max Planck InstituteContact: Maren Berghoff – Max Planck InstituteImage: The image is credited to MPI f. Biology of Ageing/ S. Deshwal

Original Research: Open access."Mitochondria regulate intracellular coenzyme Q transport and ferroptotic resistance via STARD7" by Soni Deshwal. Nature Cell Biology

Abstract

Mitochondria regulate intracellular coenzyme Q transport and ferroptotic resistance via STARD7

Coenzyme Q (or ubiquinone) is a redox-active lipid that serves as universal electron carrier in the mitochondrial respiratory chain and antioxidant in the plasma membrane limiting lipid peroxidation and ferroptosis.

Mechanisms allowing cellular coenzyme Q distribution after synthesis within mitochondria are not understood.

Here we identify the cytosolic lipid transfer protein STARD7 as a critical factor of intracellular coenzyme Q transport and suppressor of ferroptosis.

Dual localization of STARD7 to the intermembrane space of mitochondria and the cytosol upon cleavage by the rhomboid protease PARL ensures the synthesis of coenzyme Q in mitochondria and its transport to the plasma membrane.

While mitochondrial STARD7 preserves coenzyme Q synthesis, oxidative phosphorylation function and cristae morphogenesis, cytosolic STARD7 is required for the transport of coenzyme Q to the plasma membrane and protects against ferroptosis.

A coenzyme Q variant competes with phosphatidylcholine for binding to purified STARD7 in vitro. Overexpression of cytosolic STARD7 increases ferroptotic resistance of the cells, but limits coenzyme Q abundance in mitochondria and respiratory cell growth.

Our findings thus demonstrate the need to coordinate coenzyme Q synthesis and cellular distribution by PARL-mediated STARD7 processing and identify PARL and STARD7 as promising targets to interfere with ferroptosis.